Determination of Linkages in β-d-Glucans From Phaseolus aureus by exo-β-(1→3)-d-Glucanase
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چکیده
منابع مشابه
Leukocyte activation by (1→3)-β-D glucans
We studied the activities of several kinds of beta-glucans, including sonifilan, grifolan, Sclerotinia sclerotiorum glucan, laminarin and zymosan, on macrophages. Preculture of macrophages with inactive beta-glucans rendered the cells unresponsive to subsequent stimulation with grifolan, suggesting a specific pathway in the beta-glucan structure. The importance of protein C and phosphorylation ...
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Rodjana OPASSIRI*, Yanling HUA†, Onnop WARA-ASWAPATI*, Takashi AKIYAMA‡, Jisnuson SVASTI§, Asim ESEN¶ and James R. KETUDAT CAIRNS*1 *Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand, †Center for Scientific and Technological Equipment, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand, ‡Department of Low Temperature Science, National...
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Particulate, and digitonin-solubilized, enzyme systems from Phaseolus aureus and Lupinus albus catalyze the biosynthesis of aqueous-insoluble glucans from UDP-d-glucose. The digitonin treatment greatly increases the enzymic activity of (per unit protein) both the 34,000g pellet and the supernatant liquid as compared with that of the original particles. Most of the polymer produced (90-95%) is s...
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An entomopathogenic fungus, Paecilomyces lilacinus, was found to grow on chitosanase-detecting plates. Besides an endo-chitosanase, an exo-β-D-glucosaminidase was purified by cation-exchange chromatography from this microorganism cultivated in M9 minimal media containing 0.5% chitosan as the sole carbon source. The molecular weight of the enzyme is 95kDa; the optimum pH and temperature for acti...
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β-Glucans have been known to exhibit antitumor activities by potentiating host immunity by an unknown mechanism. The C-type lectin dectin-1, a β-glucan receptor, is found on the macrophage and can recognize various β-glucans. Previously, we demonstrated the presence of β-glucan receptor, dectin-1, on the Raw 264.7 cells as well as on murine mucosal organs, such as the thymus, the lung, and the ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1969
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.44.5.755